Download e-book for iPad: Advances in lipid research. / Volume 19 by Rodolfo Paoletti, Dr. David Kritchevsky

By Rodolfo Paoletti, Dr. David Kritchevsky

ISBN-10: 0120249197

ISBN-13: 9780120249190

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1978). The changes in glycosaminoglycan metabolism associated with a direct effect of lipoproteins, however, are far from being understood, and it seems that in vitro experiments using isolated lipoprotein fractions are required to interpret their action at the cellular level. , 1979; Wight, 1980). Not only smooth muscle cell metabolism of GAG could be affected by atherogenesis and its associated lipoprotein deposition. Likar et al. (1981) have demonstrated that mast cells in the arterial intima-media contribute to the net metabolism of GAG; and Merrilees and Scott (1981a,b) have found that intraspecific interactions of endothelial and smooth muscle cells affect markedly the biosynthesis of GAG, increasing HA and to a smaller extent sulfated GAG.

Differential scanning calorimetry of LDL solutions showed a reversible transition at 33°C, with a small shoulder at 38°C. Therefore, at body temperature, LDL cholesteryl esters, the main contributors to the transition, exist in a liquid state. When LDL was mixed with GAG, only C-6-S affected the transition temperature, raising it to 40°C. If such associations take place in vivo, they could cause the presence of LDL cholesteryl esters in a mesomorphic liquid crystalline state at 37°C. Such change, if taking place in the arterial wall extracellular matrix, may have profound effects on the mobilization and catabolism of LDL particles.

VLDL 34 GERMAN CAMEJO and LDL are bound to both gels; however, L p (a) lipoprotein, a particle that has been shown to occur frequently in coronary patients, does not bind to the gels. The lack of binding is attributed to the lower positive charge of the L p (a), as compared to LDL. 6, is bound to the GAG-agarose gels under the same conditions. With the actual knowledge about the structural specificity of arterial proteoglycans, it should be most interesting to repeat Iverius's experiments with intima-medial GAG and proteoglycans.

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Advances in lipid research. / Volume 19 by Rodolfo Paoletti, Dr. David Kritchevsky


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